수산화인회석 캐리어에 코팅된 골형성단백 2형이 토끼 두개골 골재생 모델에서 인접한 조직에 미치는 영향
Influence of BMP-2-loaded hydroxyapatite carrier on a neighboring ectopic site in the rabbit calvarial defect model
이재홍, 정정훈, 김유경, 이중석, 김영택, 방은경, 최성호,
소속 상세정보
이재홍 ( Lee Jae-Hong ) - 연세대학교 치과대학 치주과학교실
정정훈 ( Chung Chung-Hoon ) - 연세대학교 치과대학 치주과학교실
김유경 ( Kim You-Kyoung ) - 연세대학교 치과대학 치주과학교실
이중석 ( Lee Jung-Seok ) - 연세대학교 치과대학 치주과학교실
김영택 ( Kim Young-Taek ) - 국민건강보험공단 일산병원 치주과
방은경 ( Pang Eun-Kyoung ) - 이화여자대학교 의학전문대학원 치주과학교실
최성호 ( Choi Seong-Ho ) - 연세대학교 치과대학 치주과학교실
KMID : 1034220150070010005
Abstract
The aim of this study was to elucidate the influence of bone morphogenetic protein-2 (BMP-2)-loaded hydroxyapatite (HA) carrier on a neighboring surgical defect in the rabbit calvarial defect model. Two circular bicortical defects (8 mm in diameter) were created 2 mm apart on each rabbit calvarium (n=10 animals in each group). The rabbits were randomly divided into two groups: BMP-2-loaded HA and HA only. Control defects were designated as HAC (the sham surgical site located at the neighboring defect filled with HA only) and HAB (the sham surgical site located at the neighboring defect filled with BMP-2-loaded HA). Twenty animals were sacrificed at 2 and 8weeks postsurgery, and microcomputed tomographic, histologic, and histomorphometric evaluations were performed. The HAC and HAB groups showed small amounts of new bone formation at both healing periods. Radiographic, histologic, and histomorphometric features such as defect closure, new bone area, total augmented area, total bone/new bone volume, and closure volume did not differ significantly between the two groups. When evaluating the influence of BMP-2-loaded HA on a neighboring sham surgical site, inadvertent BMP-2 expression will not occur when the circular defects are separated by at least 2 mm.
키워드
Bone graft; Bone morphogenetic protein-2; Bone regeneration
원문 및 링크아웃 정보
등재저널 정보